1. 05 mol/L hydrochloric acid and 0. 7. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax ABS Plus SpectraTest ABS2 v1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 7°C (gradient heating design). SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Background-subtracted data values (mean +/- SD) are graphed. Results and Discussion Small-molecule agonists have been identified for TLR7 [ 41 , 42 , 47 , 48 , 60 – 67 ], TLR8 [ 49 , 51 – 54 , 68 ], TLR4 [ 69 – 72 ] and TLR2 [ 73 , 74 ]. Following incubation, the media was aspirated completely and MTT formazan crystals formed were dissolved by the addition of dimethylsulfoxide, and the reading was taken at 570 nm using SpectraMax M4. . SpectraMax® L (software version 5. C. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. Table of Contents. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Background-subtracted data values (mean +/- SD, n =2) are graphed. SpectraMax® iD5. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. $2,219. SpectraMax M Series Multi-Mode Microplate Readers | MolecularThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Table 2. 11. Bioz Stars score: 98/100, based on 1 PubMed citations. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. com. C. Dagda Lab. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Highly selective for dsDNA over ssDNA or RNA. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. FP assay do not perform well with protein:protein interactions. 4. Category 2 and 3 microplate readers use gradient heating as well. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. 05 mg mL −1. 10. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The IP-10 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax® M4). Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. 3, La Jolla, CA, USA). The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. Description 1. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. After aerosol collection was completed, the impactor plates were rinsed with 0. SpectraMax® M4). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). However, dynamic changes in soil microbiota under different tree ages have received little attention. 2. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. In. . The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. Examples: “Press carefully to engage,” “ Do not press down. Viability was determined by recording absorbance at 570 nm with plate reader (SpectraMax M4 Multimode, USA) and cell viability was determined. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). Where is the instrument serial number located? The serial number is on. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. 116 SpectraMax M5 and M5e Multi-Mode. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). Images were visualized using a fluorescence microscope (Nikon, Japan) . 2. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. Then 10 µL of CCK-8 reagent was added to each well, and cell growth was measured at 490 nm using the SpectraMax M4 Multimode Microplate reader (Molecular Devices, San Jose, CA, USA). VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. spr SpectraMax Plus 384. 161 likes. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Measurement of Mitochondrial Membrane Potential by JC-1. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. For SpectraMax L or LMax II, LMax II 384 - no adapter used. , cyan fluorescence at 458 ex. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 7% of the total test aerosol mass) was not included in the collection analysis. When a feature or capability applies to only certain readers, this exception is noted. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. Background-subtracted data values (mean +/- SD) are graphed. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. 0 compensates for most changes in environmental temperature or pressure. Results were expressed as a percentage relative to the control group (defined as 100%). SPECTROMAXx with iCAL 2. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. Following digestion, 200 μl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485- and 525-nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). SpectraMax Gemini XPS/EM Register or Sign In. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. D 47 7. Click Read. Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 2. Like all. All experiments. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Lysosomal proteolytic activity. Background-subtracted data values (mean +/- SD) are graphed. Three kits covering a wide range of dsDNA concentrations. Flasks containing 100 mL of YM broth were inoculated with 100. The plate reader. free. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. , and red fluorescence at 543 ex. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. $1,250. Introduction. Our absorbance plate readers feature our PathCheck Sensor. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Background-subtracted data values (mean +/- SD) are graphed. Results. 73. High tolerance for contaminants. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SpectraMax® M5. 4) In vivo pharmacokinetic studies. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. The sample absorbance was read at 450 nm using a SpectraMax M4 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. 303 unable to cal dark current. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). All experiments. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Other technologies such as imaging. We would like to show you a description here but the site won’t allow us. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Three kits covering a wide range of dsDNA concentrations. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. The MDD was determined by calculating the mean of zero standard replicates (n=24) and. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 1. The cleaved substrate was excited at 356-nm, and the fluorescence emissions were detected. The SpectraMax M2 and M2e are versatile and reliable. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. Data was calculated subtracting OD440 recorded with the final OD600 values. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. SpectraMax® ABS Plus. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). Kinetic fluorescence measurements were taken for 45 min at 37 °C in a SpectraMax M4 microplate reader (540 nm ex /590 nm em). 5. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 3 or greater. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. reader (SpectraMax M4, Molecular Devices; Sunnyvale, CA, USA) at 540 nm absorbance. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Add To My List; Create New List; Quickview. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. FlexStation™ I. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. 6 mm). Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. Microplate Reader Shelf. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. Laboratory Freezers. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. StakMax Handling Stacker Base Unit. The test conditions are as follows: mobile phase: acetonitrile/H2O (0. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. M4 Contracting Ltd, Victoria, British Columbia. Background-subtracted data values (mean +/- SD) are graphed. To report an issue with this product, Brief content visible, double tap to read full content. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 0. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Full content visible, double tap to read brief content. Standard features include a cuvette port,. 5 mg mL −1 with 0. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Microscopic evaluation of apoptosis. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. TEM. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. All associated StakMax® stackers. Assessment of Neurite Outgrowth. Published Dec 16, 2020 | Updated Dec 16, 2020. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. book Page 1 Wednesday, July 7, 2010 2:09 PM. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. . STAKMAX. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. org) with optimized instrument settings (Table 1) were used to generate and analyze data. , 2020b). Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Table of Contents. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. , contact Molecular. The combination index (CI) was calculated. Serial number location on Series II and Multi-Mode plate readers. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. D 47 7. Background-subtracted data values (mean +/- SD) are graphed. of a 96 well-plate and cultured for 24h at 37 °C. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. overnight. 8. All configurations. 03 in x 3. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. Data analysis methods. Other technologies such as imaging. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The data represent the mean ± SD of three independent experiments. B07F4876MS. NCI-60 Human Tumor Cell Line Screen. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Background-subtracted data values (mean +/- SD) are graphed. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. VIEW DETAILS. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. 0397 in x 3. 5 mg mL −1 with 0. Next to the pushbutton is a green LED. 1 & 7. , San Jose, CA) was. SpectraMax M3, M4, and M5/M5e readers. Highly selective for dsDNA over ssDNA or RNA. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Ltd. We therefore gathered salient. Background-subtracted data values (mean +/- SD) are graphed. Lift up the lid over the chamber to insert or remove a cuvette. 05 mg mL −1. Results were expressed as the mean percentage of CV compared with NC group. Instruments Ltd. Exhibit with us {{>currencyLabel}} Back. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Cellular Reactive Oxygen Species (ROS) was measured in Neuro2a cells seeded in 6-well plate at a density of 5000 cells/well. Cell uptake studies. Roelli1, Amandine Stoss1, Rebecca A. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. 304 signal level saturation. Bioz Stars score: 86/100, based on 1 PubMed citations. For a complete list of the instruments supported by this release of the SoftMax. Table 2. . Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 0 statistics software. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. ® reader to the computer. Italic and boldface type are used for emphasis. The combination index (CI) was calculated. 1. 1 Figure from 528 Publications Molecular Devices LLC. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. Cells were seeded at 5000 cells well −1 on 96-well plates and allowed to grow for 24 h before treatment. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. Statistical analysis. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. 315 can't find zero order. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 1 Measurement of Intestinal Permeability (In Vivo). SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. 2. Multiplate reader for ELISA detection (Spectramax M4). In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). Data are from ≥5 independent experiments. Cells were exposed to drug solutions with. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. Cet appareil ISM est confomre à la norme NMB-001 du Canada. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). 4. All configurations. to View Figures. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. † Bottom mounting for SpectraMax Paradigm Readers only. D 1 1. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. spr * SpectraMax i3(x) SpectraTest ABS2 v1. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. l. Background-subtracted data values (mean +/- SD) are graphed. All configurations. .